Recirculating Aquaculture Systems - RAS

Nitrifying Bacterial Consortium

Removal of Ammonia and Nitrite from Aquaculture Tanks is a key component in development of a Recirculating Aquaculture Systems (RAS). This is accomplished in the Nitrifying Bioreactor using a specific Nitrifying Bacterial Consortium.

Nitrifying bioreactors used for the establishment of Recirculating Aquaculture Systems (RAS) are activated with consortia of nitrifiers by immobilizing them on an inert substratum designed as cartridge. They grow as biofilm and as water passes over nitrification is effected. The consortia consist of diverse flora of autotrophic nitrifiers as presented in the figures below.

NsV 443 (Nitrosospira sp.)
NsV 443 (Nitrosospira sp.)

Nitrifiers inside cysts
Nitrifiers inside cysts

 NIT2 (Nitrobacter sp.)
NIT2 (Nitrobacter sp.)

Ntspa 712 (Phylum Nitrospira)
Ntspa 712 (Phylum Nitrospira)

Autotrophic Nitrifiers
Cells having intracytoplasmic membranes and carboxysomes characteristic to the autotrophic nitrifiers

 NmV (Nitrosococcusmobilislinneage)
NmV (Nitrosococcusmobilislinneage)

 DAPI stained consortia
DAPI stained consortia

Diversity of the cells in the consortia
Diversity of the cells in the consortia

EUB 338 (Bacteria)
EUB 338 (Bacteria)

 NSO 190 (β Ammonia Oxidizers)
NSO 190 (β Ammonia Oxidizers)

NEU (Halophilic Nitrosomonas sps)
NEU (Halophilic Nitrosomonas sps)

The bacterial consortium is maintained separately for fresh water, brackish water and marine water conditions. Different bacterial consortia are used for establishment of Recirculating Aquaculture Systems (RAS) for fresh water, brackish water and marine water conditions.

Why Recirculating Aquaculture Systems

Aquaculture hatcheries currently use the open larval rearing system. The open larval rearing systems have been found to have certain shortcomings. These are illustrated in the figure below and explained subsequently.

In an open larval rearing system in a hatchery, there is continuous exchange of seawater on a regular basis. The quantum of exchange varies depending on the hatchery and could be as high as 100%. Due to the heavy demand of seawater in the production...

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Recirculating Aquaculture Systems (RAS)

Recirculating aquaculture systems, or RAS, are closed-loop production systems that continuously filter and recycle water, enabling large-scale fish farming that requires a small amount of water and releases little or no pollution.

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